Normal Human Epidermal Melanocytes 2 (NHEM 2)

Producer

Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs.
Melanocytes are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes.
The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.
PromoCell Normal Human Epidermal Melanocytes 2 are isolated using the serum-free and PMA-free Melanocyte Growth Medium M2. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M2 and also using this medium for cultivation.
Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.

 
 
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Article No. Description Cell Type Donor Organ Donor Preparation Unit/Case
Cells
On Stock
C-12402-PRO NHEM juvenile foreskin, cult. in M2 Medi NHEM juvenile foreskin, cult. in M2 Medium, cryopreserved Human Epidermal Melanocytes derived from foreskin   cultured in M2 medium, cryopreserved 500000 Delivery time
C-12403-PRO NHEM adult donor, cultured in M2 Medium, NHEM adult donor, cultured in M2 Medium, cryopreserved Human Epidermal Melanocytes derived from adult skin adult cultured in M2 medium, cryopreserved 500000 Delivery time
C-12452-PRO NHEM juvenile foreskin, cult. in M2 Medi NHEM juvenile foreskin, cult. in M2 Medium, proliferating Human Epidermal Melanocytes derived from foreskin   cultured in M2 medium, proliferating 500000 Delivery time
C-12453-PRO NHEM adult donor, cultured in M2 Medium, NHEM adult donor, cultured in M2 Medium, proliferating Human Epidermal Melanocytes derived from adult skin adult cultured in M2 medium, proliferating 500000 Delivery time
C-14042-PRO NHEM M2 Pellet, in RNAlater NHEM M2 Pellet, in RNAlater Normal Human Epidermal Melanocytes from foreskin   cultured in M2 medium, Pellet 1000000 Delivery time
C-14043-PRO NHEM M2 adult Pellet, in RNAlater NHEM M2 adult Pellet, in RNAlater Normal Human Epidermal Melanocytes from adult skin adult cultured in M2 medium, Pellet 1000000 Delivery time
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Freezing Medium Cryo-SFM Freezing Medium Cryo-SFM
Melanocyte Medium M2 Melanocyte Medium M2
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